Comprehensive Physiology Wiley Online Library

Intracellular Digestion: Lysosomes and Cellular Injury

Full Article on Wiley Online Library



Abstract

The sections in this article are:

1 Origin and Function of Lysosomes
2 Lysosomes and Tissue Injury
2.1 Lysosomal Enzymes
2.2 Autophagy
2.3 Mechanisms of Lysosomal Enzyme Release
2.4 Interactions of Lysosomes With Chemical and Physical Agents
3 Physical Agents
3.1 Ionizing Radiation
3.2 Ultraviolet Radiation
3.3 Visible Radiation
4 Microbes and Microbial Products
4.1 Bacteria and Fungi
4.2 Microbial Toxins
4.3 Viruses
5 Chemical Agents
5.1 Drugs, Vitamins, and Hormones
6 Heavy Metals
7 Environmental Pollutants
8 Venoms and Toxins
References
 1. Abraham, R., and R. J. Hendy. Irreversible lysosomal damage induced by chloroquine in the retinae of pigmented and albino rats. Exptl. Mol. Pathol. 12: 185–200, 1970.
 2. Abraham, R., R. Hendy, and P. Grasso. Formation of myeloid bodies in rat liver lysosomes after chloroquine administration. Exptl. Mol Pathol. 9: 212–229, 1968.
 3. Alexander, P., and Z. M. Bacq. The nature of the initial radiation damage at the sub‐cellular level. In: Initial Effects of Ionizing Radiations on Cells, edited by R. J. C. Harris. London: Academic Press, 1961, p. 3–19.
 4. Allison, A. C. Role of lysosomes in oxygen toxicity. Nature 205: 141–143, 1965.
 5. Allison, A. C. Lysosomes in virus infected cells. Perspectives Virol. 5: 29–37, 1967.
 6. Allison, A. C. Lysosomes and the toxicity of particulate pollutants. Arch. Internal Med. 128: 131–139, 1971.
 7. Allison, A. C., J. S. Harington, and M. Birbeck. An examination of the cytotoxic effects of silica on macrophages. J. Exptl. Med. 124: 141–154, 1968.
 8. Allison, A. C., I. A. Magnus, and M. R. Young. Role of lysosomes and of cell membranes in photosensitization. Nature 209: 874–878, 1966.
 9. Allison, A. C., and L. Mallucci. Histochemical studies of lysosomes and lysosomal enzymes in virus‐infected cell cultures. J. Exptl. Med. 121: 463–476, 1965.
 10. Allison, A. C., and K. Sandelin. Activation of lysosomal enzymes in virus‐infected cells and its possible relationship to cytopathic effects. J. Exptl. Med. 117: 879–887, 1963.
 11. Allison, A. C., and M. R. Young. Uptake of dyes and drugs by living cells in culture. Life Sci. 3: 1407–1414, 1964.
 12. Allison, A. C., and M. R. Young. Vital staining and fluorescence microscopy of lysosomes. In: Frontiers of Biology: Lysosomes in Biology and Pathology, edited by J. T. Dingle and H. B. Fell. Amsterdam: North‐Holland, 1969, vol. 14, pt. 2, p. 600–628.
 13. Avila, J. L., and J. Convit. Studies on human polymorphonuclear leukocyte enzymes. I. Assay of acid hydrolases and other enzymes. Biochim. Biophys. Acta 293: 397–408, 1973.
 14. Bablanian, R., H. J. Eggers, and I. Tamm. Studies on the mechanism of poliovirus‐induced cell damage. I. The relation between poliovirus‐induced metabolic and morphological alterations in cultured cells. Virology 26: 100–113, 1965.
 15. Baehner, R. L., M. J. Karnovsky, and M. L. Karnovsky. Degranulation of leukocytes in chronic granulomatous disease. J. Clin. Invest. 48: 187–192, 1969.
 16. Baggiolini, M. The enzymes of the granules of polymorphonuclear leukocytes and their functions. Enzyme 13: 132–160, 1972.
 17. Bainton, D. F., and M. G. Farquhar. Origin of granules in polymorphonuclear leukocytes. Two types derived from opposite faces of the Golgi complex in developing granulocytes. J. Cell Biol. 28: 277–301, 1966.
 18. Bainton, D. F., J. L. Ullyot, and M. G. Farquhar. The development of neutrophilic polymorphonuclear leukocytes in human bone marrow. Origin and content of azurophil and specific granules. J. Exptl. Med. 134: 907–934, 1971.
 19. Bangham, A. D., M. M. Standish, and G. Weissmann. The action of steroids and streptolysin S on the permeability of phospholipid structures to cations. J. Mol. Biol. 13: 253–259, 1965.
 20. Bastos, A. L., A. M. Terrinha, J. D. Vigario, J. F. Moura Nunes, and J. L. Nunes Petisca. U‐V induced changes in lysosomes tagged with quinacrine. Exptl. Cell Res. 42: 84–88, 1966.
 21. Bernheimer, A. W., and L. L. Schwartz. Lysosomal disruption by bacterial toxins. J. Bacteriol. 87: 1100–1104, 1964.
 22. Bernstein, H., N. Zvaifler, M. Rubin, and A. M. Mansour. The ocular deposition of chloroquine. Invest. Ophthalmol. 2: 384–392, 1963.
 23. Blackman, K. E., and H. C. Bubel. Poliovirus‐induced cellular injury. J. Virol. 4: 203–208, 1969.
 24. Brun, A., and U. Brunk. Histochemical indications for lysosomal localization of heavy metals in normal rat brain and liver. J. Histochem. Cytochem. 18: 820–827, 1970.
 25. Brunning, R. D., B. F. Woolfrey, and W. H. Schrader. Studies with tritiated endotoxin. II. Endotoxin localization in the formed elements of the blood. Am. J. Pathol. 44: 401–409, 1964.
 26. Burnett, J. W., and W. M. Gould. Further studies on the purification and physiological actions of sea nettle toxin. Proc. Soc. Exptl. Biol. Med. 138: 759–762, 1971.
 27. Caffey, J. Chronic poisoning due to excess of vitamin A; description of the clinical and roentgen manifestations in seven infants and young children. Pediatrics 25: 508–515, 1950.
 28. Caro, L. G., and G. E. Palade. Protein synthesis, storage, and discharge in the pancreatic exocrine cell. An autoradiographic study. J. Cell Biol. 20: 473–495, 1964.
 29. Cline, M. J., K. L. Melmon, W. C. Davis, and H. E. Williams. Mechanism of endotoxin interaction with human leucocytes. Brit. J. Haematol. 15: 539–547, 1968.
 30. Cohn, Z. A. The structure and function of monocytes and macrophages. Advan. Immunol. 9: 163–214, 1968.
 31. Cohn, Z. A., and B. Benson. The in vitro differentiation of mononuclear phagocytes. I. The influence of inhibitors and the results of autoradiography. J. Exptl. Med. 121: 279–288, 1965.
 32. Cohn, Z. A., and B. Benson. The in vitro differentiation of mononuclear phagocytes. II. The influence of serum on granule formation, hydrolase production, and pinocytosis. J. Exptl. Med. 121: 835–848, 1965.
 33. Cohn, Z. A., and M. E. Fedorko. The formation and fate of lysosomes. In: Frontiers of Biology: Lysosomes in Biology and Pathology, edited by J. T. Dingle and H. B. Fell. Amsterdam: North‐Holland, 1969, vol. 14, pt. 1, p. 43–63.
 34. Cohn, Z. A., M. E. Fedorko, and J. G. Hirsch. The in vitro differentiation of mononuclear phagocytes. V. The formation of macrophage lysosomes. J. Exptl. Med. 123: 757–766, 1966.
 35. Cohn, Z. A., and J. G. Hirsch. Isolation and properties of specific cytoplasmic granules of rabbit polymorphonuclear leucocytes. J. Exptl. Med. 112: 983–1004, 1960.
 36. Davies, P., R. I. Fox, M. Polyzonis, A. C. Allison, and A. D. Haswell. The inhibition of phagocytosis and facilitation of exocytosis in rabbit polymorphonuclear leukocytes by cytochalasin B. Lab. Invest. 28: 16–22, 1973.
 37. De Duve, C., The lysosome concept. In: Lysosomes, edited by A. V. S. deReuck and M. P. Cameron. Boston: Little, Brown, 1963, p. 1–31. (Ciba Foundation Symposium.).
 38. De Duve, C., B. C. Pressman, R. Gianetto, R. Wattiaux, and F. Appelmans. Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat liver tissue. Biochem. J. 60: 604–617, 1955.
 39. De Duve, C., and R. Wattiaux. Functions of lysosomes. Ann. Rev. Physiol. 28: 435–492, 1966.
 40. De Duve, C., R. Wattiaux, and M. Wibo. Effect of fat soluble compounds on lysosomes in vitro. Biochem. Pharmacol. 9: 97–116, 1962.
 41. Desai, I. D., P. L. Sawant, and A. L. Tappel. Peroxidative and radiation damage to isolated lysosomes. Biochim. Biophys. Acta 86: 277–285, 1964.
 42. Dingle, J. T. Studies on the mode of action of excess of vitamin A. 3. The release of a bound protease by the action of vitamin A. Biochem. J. 79: 509–512, 1961.
 43. Dingle, J. T. Vacuoles, vesicles, and lysosomes. Brit. Med. Bull. 24: 141–145, 1968.
 44. Dingle, J. T., and A. J. Barrett. The uptake of biologically active substances by lysosomes. Proc. Roy. Soc., London, Ser. B. 173: 85–93, 1969.
 45. Dingle, J. T., and A. J. Barrett. Uptake of biologically active substances by lysosomes. Proc. Roy. Soc., London, Ser. B. 173: 85–93, 1969.
 46. Ennis, R. S., J. L. Granda, and A. S. Posner. Effect of gold salts and other drugs on the release and activity of acid hydrolases. Arthritis Rheumat. 11: 756–764, 1968.
 47. Ericsson, J. L. E., Mechanisms of cellular autophagy. In: Frontiers of Biology: Lysosomes in Biology and Pathology, edited by J. T. Dingle and H. B. Fell. Amsterdam: North‐Holland, 1969. vol. 14, pt. 2, p. 345–394.
 48. Fedorko, M. Effect of chloroquine on morphology of cytoplasmic granules in maturing human leukocytes—an ultrastructural study. J. Clin. Invest. 46: 1932–1942, 1967.
 49. Fedorko, M. E., and J. G. Hirsch. Cytoplasmic granule formation in myelocytes. An electron microscope radioautographic study on the mechanism of formation of cytoplasmic granules in rabbit heterophilic myelocytes. J. Cell Biol. 29: 307–316, 1966.
 50. Fell, H. B., and J. T. Dingle. Studies on the mode of action of excess vitamin A. 6. Lysosomal protease and the degradation of cartilage matrix. Biochem. J. 87: 403–408, 1963.
 51. Filkins, J. P. Comparison of endotoxin detoxification by leukocytes and macrophages. Proc. Soc. Exptl. Biol. Med. 137: 1396–1400, 1971.
 52. Gillespie, E., and L. M. Lichtenstein. Histamine release from human leucocytes: Studies with deuterium oxide, colchicine and cytochalasin B. J. Clin. Invest. 51: 2941–2947, 1972.
 53. Goldberg, J., L. E. Martini, and A. Batchelor. Biochemical changes in the tissues of animals injected with iron, acid phosphatase and other enzymes. Biochem. J. 77: 252–262, 1960.
 54. Goldfischer, S. The localization of copper in the pericanalicular granules (lysosomes) of liver in Wilson's disease (hepatolenticular degeneration). Am. J. Pathol. 46: 977–983, 1965.
 55. Goldstein, I., S. Hoffstein, J. Gallin, and G. Weissmann. Mechanisms of lysosomal enzyme release from human leukocytes: Microtubule assembly and membrane fusion induced by a component of complement. Proc. Natl. Acad. Sci. US 70: 2916–2920, 1973.
 56. Gordon, G. B., L. R. Miller, and K. G. Bensch. Studies on the intracellular digestive process in mammalian tissue culture cells. J. Cell Biol. 25: 41–56, 1965.
 57. Gottlieb, D., H. E. Carter, L. Wu, and J. H. Sloneker. Inhibition of fungi by filipin and its antagonism by sterols. Phytopathology 50: 594–603, 1960.
 58. Gotze, O., and H. J. Muller‐Eberhard. The C3‐activator system: an alternate pathway of complement activation. J. Exptl. Med. 134: 90s–108s, 1971.
 59. Goutier‐Pirotte, M., and R. Goutier. Acid deoxyribonu‐clease and acid phosphatase activities in regenerating rat liver after whole‐body X‐irradiation. Radiation Res. 16: 728–735, 1962.
 60. Guskey, L. E., P. C. Smith, and D. A. Wolff. Patterns of cytopathology and lysosomal enzyme release in poliovirus‐infected HEp‐2 cells treated with either 2‐(α‐hydroxybenzyl)‐benzimidazole or guanidine HCl. J. Gen. Virology 6: 151–161, 1970.
 61. Hall, I. H., and A. P. Sanders. Effects of hyperbaric oxygenation on metabolism. III. Succinic dehydrogenase, acid phosphatase, cathepsin and soluble nitrogen. Proc. Soc. Exptl. Biol. Med. 121: 1203–1206, 1966.
 62. Hara, K., and T. Nozima. In vitro reaction between lysosomes and influenza virus. Exptl. Cell Res. 72: 361–369, 1972.
 63. Harris, J. W. Factors influencing the apparent radiosensitivity and heat stability of isolated leucocyte lysosomes. Intern. J. Radiation Biol. 11: 465–476, 1966.
 64. Harris, J. W. Binding of sulfhydryl reagents to normal and irradiated lysosomes. Australian J. Exptl. Biol. Med. Sci. 46: 209–217, 1968.
 65. Harris, J. W. Effects of ionizing radiation on lysosomes and other intracellular membranes. Advan. Biol. Med. Phys. 13: 273–287, 1970.
 66. Hawkins, D. Neutrophilic leucocytes in immunologic reactions: Evidence for the selective release of lysosomal constituents. J. Immunol. 108: 310–318, 1972.
 67. Hendy, R. J., R. Abraham, and P. Grasso. The effect of chloroquine on rat heart lysosomes. J. Ultrastruct. Res. 29: 485–495, 1969.
 68. Henson, P. M. The immunologic release of constituents from neutrophil leucocytes. II. Mechanisms of release during phagocytosis and adherence to nonphagocytosable surfaces. J. Immunol. 107: 1547–1557, 1971.
 69. Henson, P. M. Interaction of cells with immune complexes: Adherence, release of constituents, and tissue injury. J. Exptl. Med. 134: 114s–135s, 1971.
 70. Hers, H. G., and F. van Hoof. Genetic abnormalities of lysosomes. In: Frontiers of Biology: Lysosomes in Biology and Pathology, edited by J. T. Dingle and H. B. Fell. Amsterdam: North‐Holland, 1969, vol. 14, pt. 2, p. 19–40.
 71. Hill, D. F. Gold therapy for rheumatoid arthritis. Med. Clin. N. Am. 52: 733–746, 1968.
 72. Hirsch, J. G., A. W. Bernheimer, and G. Weissmann. Motion picture study of the toxic action of streptolysins on leucocytes. J. Exptl. Med. 118: 223–228, 1963.
 73. Hugon, J., J. R. Maisin, and M. Borgers. Lysosomal modifications after administration of radioprotective drugs. Experientia 22: 235–236, 1966.
 74. Hurst, D. J., and D. L. Coffin. Ozone effect on lysosomal hydrolases of alveolar macrophages in vitro. Arch. Internal Med. 127: 1059–1063, 1971.
 75. Ignarro, L. J. Effects of anti‐inflammatory drugs on the stability of rat liver lysosomes in vitro. Biochem. Pharmacol. 20: 2847–2860, 1971.
 76. Inglot, A. D., and E. Wolna. Reactions of non‐steroidal anti‐inflammatory drugs with the erythrocyte membrane. Biochem. Pharmacol. 17: 269–279, 1968.
 77. Janoff, A. Inhibition of human granulocyte elastase by gold sodium thiomalate. Biochem. Pharmacol. 19: 626–628, 1970.
 78. Janoff, A., and R. T. McCluskey. Effect of excess vitamin A on acid phosphatase content of guinea pig peritoneal leucocytes. Proc. Soc. Exptl. Biol. Med. 110: 586–589, 1962.
 79. Janoff, A., G. Weissmann, B. W. Zweifach, and L. Thomas. Pathogenesis of experimental shock. IV. Studies on lysosomes in normal and tolerant animals subjected to lethal trauma and endotoxemia. J. Exptl. Med. 116: 451–466, 1962.
 80. Johnson, B. E. Ultraviolet radiation and lysosomes in skin. Nature 219: 1258–1259, 1968.
 81. Kaliner, M., R. P. Orange, and K. F. Austen. Immunologic release of histamine and slow reacting substance of anaphylaxis from human lung. IV. Enhancement by cholinergic and alpha‐adrenergic stimulation. J. Exptl. Med. 136: 556–567, 1972.
 82. Korn, E. D. Structure of biological membranes. Science 153: 1491–1498, 1966.
 83. Lampen, J. O., and P. Arnow. Significance of nystatin uptake for its antifungal action. Proc. Soc. Exptl. Biol. Med. 101: 792–797, 1959.
 84. Lewis, D. A. The actions of some non‐steroidal drugs on lysosomes. J. Pharm. Pharmacol. 22: 909–912, 1970.
 85. Lindquist, R. R. Studies on the pathogenesis of hepatolenticular degeneration. III. The effect of copper on rat liver lysosomes. Am. J. Pathol. 53: 903–927, 1968.
 86. Lucy, J. A., Lysosomal membranes. In: Frontiers of Biology: Lysosomes in Biology and Pathology, edited by J. T. Dingle and H. B. Fell. Amsterdam: North‐Holland, 1969, vol. 14, pt. 2, p. 313–341.
 87. Lucy, J. A. The fusion of biological membranes. Nature 227: 815–817, 1970.
 88. Mandell, G. L., W. Rubin, and E. W. Hook. The effect of an NADH oxidase inhibitor (hydrocortisone) on polymorphonuclear leukocyte bactericidal activity. J. Clin. Invest. 49: 1381–1388, 1970.
 89. Mego, J. L., and J. Barnes. Inhibition of heterolysosome formation and function in mouse kidneys by injection of mercuric chloride. Biochem. Pharmacol. 22: 373–381, 1973.
 90. Merkow, L., M. Pardo, S. M. Epstein, E. Verney, and H. Sidransky. Lysosomal stability during phagocytosis of Aspergillus flavus spores by alveolar macrophages of cortisone‐treated mice. Science 160: 79–81, 1968.
 91. Nachman, R., J. G. Hirsch, and M. Baggiolini. Studies on isolated membranes of azurophil and specific granules from rabbit polymorphonuclear leukocytes. J. Cell Biol. 54: 133–140, 1972.
 92. Norseth, T. The intracellular distribution of mercury in rat liver after a single injection of mercuric chloride. Biochem. Pharmacol. 17: 581–593, 1968.
 93. Novikoff, A. B., Lysosomes in the physiology and pathology of cells: contributions of staining methods. In: Lysosomes, edited by A. V. S. deReuck and M. P. Cameron. Boston: Little, Brown, 1963, p. 36–73. (Ciba Foundation Symposium.).
 94. Novikoff, A. B., E. Essner, and N. Quintana. Golgi apparatus and lysosomes. Federation Proc. 23: 1010–1022, 1964.
 95. Ohanian, S. H., and J. H. Schwab. Persistence of Group A streptococcal cell walls related to chronic inflammation of rabbit dermal connective tissue. J. Exptl. Med. 125: 1137–1148, 1967.
 96. Orange, R. P., W. G. Austen, and K. F. Austen. Immunological release of histamine and slow reacting substance of anaphylaxis from human lung. I. Modulation by agents influencing cellular levels of cyclic 3′,5′‐adenosine monophosphate. J. Exptl. Med. 134: 136s–148s, 1971.
 97. Palmer, R. H., and A. Kappas. Fever‐producing action of steroids. Med. Clin. N. Am. 47: 101–112, 1963.
 98. Persellin, R. H., and M. Ziff. The effect of gold salts on lysosomal enzymes of the peritoneal macrophage. Arthritis Rheumat. 9: 57–65, 1966.
 99. Rahman, Y. E., and A. Lindenbaum. Lysosome particles and subcellular distributions of polymeric tetravalent plutonium‐239. Radiation Res. 21: 575–583, 1964.
 100. Roth, J. S., J. Bukovsky, and H. J. Eichel. The effect of whole‐body X‐irradiation on the activity of some acid hydrolases in homogenates and subcellular fractions of rat spleen. Radiation Res. 16: 27–36, 1962.
 101. Ryser, H. J. Uptake of protein by mammalian cells: an underdeveloped area. The penetration of foreign proteins into mammalian cells can be measured and their functions explored. Science 159: 390–396, 1968.
 102. Scarpelli, D. G., E. J. Smith, and L. Fulmer. Cellular injury in amphotericin B induced nephropathy (Abstract). Am. J. Pathol. 44: 32a, 1964.
 103. Schanker, L. S., J. M. Johnson, and J. J. Jeffrey. Rapid passage of organic anions into human red cells. Am. J. Physiol. 207: 503–508, 1964.
 104. Schanker, L. S., P. A. Nafpliotis, and J. M. Johnson. Passage of organic bases into human red cells. J. Pharm. Exptl. Therap. 133: 325–331, 1961.
 105. Scheinberg, I., and I. Sternlieb. Wilson's disease. Ann. Rev. Med. 16: 119–134, 1965.
 106. Schwartz, S. L., and J. C. Bond. Stimulation of release of pinolysosomal contents of macrophages by nicotine. J. Pharm. Exptl. Therap. 183: 378–384, 1972.
 107. Sessa, G., J. H. Freer, G. Colacicco, and G. Weissmann. Interaction of a lytic polypeptide, melittin, with lipid membrane systems. J. Biol. Chem. 244: 3575–3582, 1969.
 108. Slater, T. F., and P. A. Riley. Photosensitization and lysosomal damage. Nature 209: 151–154, 1966.
 109. Sledge, C. B., and J. T. Dingle. Oxygen‐induced resorption of cartilage in organ culture. Nature 205: 140–141, 1965.
 110. Straus, W. Occurrence of phagosomes and phago‐lysosomes in different segments of the nephron in relation to the reabsorption, transport, digestion, and extrusion of intravenously injected horseradish peroxidase. J. Cell Biol. 21: 295–308, 1964.
 111. Strauss, B. S., and C. A. Stetson, Jr.. Studies on the effect of certain macromolecular substances on the respiratory activity of the leucocytes of peripheral blood. J. Exptl. Med. 112: 653–669, 1960.
 112. Takayama, S., and Y. Ojima. The role of lysosomes in the photosensitizing effect of 3‐methylcholanthrene on HeLa cells. Gann 59: 1–6, 1968.
 113. Tappel, A. L., P. L. Sawant, and S. Shibko. Lysosomes: distribution in animals, hydrolytic capacity and other properties. In: Lysosomes, edited by A. V. S. deReuck and M. P. Cameron. Boston: Little, Brown, 1963, p. 78–108. (Ciba Foundation Symposium.).
 114. Thacore, H., and D. A. Wolff. Activation of isolated lysosomes by poliovirus‐infected cell extracts. Nature 218: 1063–1064, 1968.
 115. Thines‐Sempoux, D. Chemical similarities between the lysosome and plasma membranes (Abstract). Biochem. J. 105: 20p–21p, 1967.
 116. Thompson, J. N., and G. A. Pitt. Vitamin A acid and hypervitaminosis A. Nature 188: 672–673, 1960.
 117. Utz, J. P. Antimicrobial therapy in systemic fungal infections. Am. J. Med. 39: 826–830, 1965.
 118. Vaughn, J., B. Bleany, and M. Williamson. The uptake of plutonium in bone marrow: a possible leukaemic risk. Brit. J. Haematol. 13: 492–502, 1967.
 119. Verity, M. A., and W. J. Brown. Hg2+‐induced kidney necrosis. Subcellular localization and structure‐linked lysosomal enzyme changes. Am. J. Pathol. 61: 57–74, 1970.
 120. Verity, M. A., and A. Reith. Effect of mercurial compounds on structure‐linked latency of lysosomal hydrolases. Biochem. J. 105: 685–690, 1967.
 121. Watkins, D. K. Effect of oxygen on the release of enzymes from lysosomes after irradiation. Brit. J. Radiol. 43: 152–153, 1970.
 122. Weissmann, G. Labilization and stabilization of lysosomes. Federation Proc. 23: 1038–1044, 1964.
 123. Weissmann, G. Studies of lysosomes. VI. The effect of neutral steroids and bile acids on lysosomes in vitro. Biochem. Pharmacol. 14: 525–535, 1965.
 124. Weissmann, G. The role of lysosomes in inflammation and disease. Ann. Rev. Med. 18: 97–112, 1967.
 125. Weissmann, G., The effects of steroids and drugs on lysosomes. In: Frontiers of Biology: Lysosomes in Biology and Pathology, edited by J. T. Dingle and H. B. Fell, Amsterdam: North‐Holland, 1969, vol. 14, pt. 1, p. 276–295.
 126. Weissmann, G., B. Becher, and L. Thomas. Studies on lysosomes. V. The effect of streptolysins and other hemolytic agents on isolated leucocyte granules. J. Cell Biol. 22: 115–126, 1964.
 127. Weissmann, G., and J. T. Dingle. Release of lysosomal protease by ultraviolet irradiation and inhibition by hydrocortisone. Exptl. Cell. Res. 25: 207–210, 1961.
 128. Weissmann, G., and H. B. Fell. The effect of hydrocortisone on the response of fetal rat skin in culture to ultraviolet irradiation. J. Exptl. Med. 116: 365–380, 1962.
 129. Weissmann, G., R. Hirschhorn, and K. Krakauer. Effect of melittin upon cellular and lysosomal membranes. Biochem. Pharmacol. 18: 1771–1775, 1969.
 130. Weissmann, G., R. Hirschhorn, M. Pras, G. Sessa, and V. A. H. Bevans. Studies on lysosomes. VIII. The effect of polyene antibiotics on lysosomes. Biochem. Pharmacol. 16: 1057–1069, 1967.
 131. Weissmann, G., and H. Keiser. Hemolysis and augmentation of hemolysis by neutral steroids and bile acids. Biochem. Pharmacol. 14: 537–546, 1965.
 132. Weissmann, G., H. Keiser, and A. W. Bernheimer. Studies on lysosomes III. The effects of streptolysins O and S on release of acid hydrolases from a granular fraction of rabbit liver. J. Exptl. Med. 118: 205–222, 1963.
 133. Weissmann, G., M. Pras, and L. Rosenberg. Arthritis induced by filipin in rabbits. Arthritis Rheumat. 10: 325–336, 1967.
 134. Weissmann, G., and G. A. Rita. Molecular basis of gouty inflammation: Interaction of monosodium urate crystals with lysosomes and liposomes. Nature New Biol. 240: 167–172, 1972.
 135. Weissmann, G., G. Sessa, and A. W. Bernheimer. Staphylococcal alpha toxin: effects on artificial lipid spherules. Science 154: 772–774, 1966.
 136. Weissmann, G., and L. Thomas. Studies on lysosomes. I. The effects of endotoxin, endotoxin tolerance, and cortisone on the release of acid hydrolases from a granular fraction of rabbit liver. J. Exptl. Med. 116: 433–450, 1962.
 137. Weissmann, G., and L. Thomas. Studies on lysosomes. II. The effect of cortisone on the release of acid hydrolases from a large granule fraction of rabbit liver induced by an excess of vitamin A. J. Clin. Invest. 42: 661–669, 1963.
 138. Weissmann, G., J. W. Uhr, and L. Thomas. Acute hypervitaminosis A in guinea pigs. I. Effects on acid hydrolases. Proc. Soc. Exptl. Biol. Med. 112: 284–287, 1963.
 139. Weissmann, G., R. B. Zurier, and S. Hoffstein. Leukocytic proteases and the immunologic release of lysosomal enzymes. Am. J. Pathol. 68: 539–559, 1972.
 140. Weissmann, G., R. B. Zurier, P. J. Spieler, and I. M. Goldstein. Mechanisms of lysosomal enzyme release from leucocytes exposed to immune complexes and other particles. J. Exptl. Med. 134: 149s–165s, 1971.
 141. Wills, E. D., and A. E. Wilkinson. Release of enzymes from lysosomes by irradiation and the relation of lipid peroxide formation to enzyme release. Biochem. J. 99: 657–666, 1966.
 142. Witschi, H. P., and W. N. Aldridge. Uptake, distribution and binding of beryllium to organelles of the rat liver cell. Biochem. J. 106: 811–820, 1968.
 143. Wolff, D. A., and H. C. Bubel. The disposition of lysosomal enzymes as related to specific viral cytopathic effects. Virology. 24: 502–505, 1964.
 144. Woodin, A. M., J. E. French, and V. T. Marchesi. Morphological changes associated with the extrusion of protein induced in the polymorphonuclear leucocyte by staphylococcal leucocidin. Biochem. J. 87: 567–571, 1963.
 145. Woodin, A. M., and A. A. Wieneke. Site of protein secretion and calcium accumulation in the polymorphonuclear leucocyte treated with leucocidin. In: Symposium on Calcium and Cellular Function, edited by A. W. Cuthbert. London: St. Martin's, 1970, p. 183–197.
 146. Yunis, A. A., and W. J. Harrington. Metabolic effects of bacterial pyrogens upon human leukocytes. I. Studies on leukocyte respiration, glycolysis, and nucleic acid synthesis. J. Lab. Clin. Med. 55: 584–592, 1960.
 147. Zucker‐Franklin, D. Electron microscope study of the degranulation of polymorphonuclear leukocytes following treatment with streptolysin. Am. J. Pathol. 47: 419–425, 1965.
 148. Zucker‐Franklin, D., and J. G. Hirsch. Electron microscope studies on the degranulation of rabbit peritoneal leukocytes during phagocytosis. J. Exptl. Med. 120: 569–576, 1964.
 149. Zurier, R. B., S. Hoffstein, and G. Weissmann. Mechanisms of lysosomal enzyme release from human leukocytes. I. Effect of cyclic nucleotides and colchicine. J. Cell Biol. 58: 27–41, 1973.
 150. Zurier, R. B., and G. Weissmann. Anti‐immunologic and anti‐inflammatory effects of steroid therapy. Med. Clin. N. Am. 57: 1295–1307, 1973.
 151. Zurier, R. B., G. Weissmann, S. Hoffstein, S. Kammerman, and H.‐H. Tai. Mechanisms of lysosomal enzyme release from human leucocytes: II. Effects of cyclic adenosine monophosphate and cyclic guanosine monophosphate, autonomic agonists and agents which affect microtubule function. J. Clin. Invest. 53: 297–309, 1974.

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How to Cite

Ira M. Goldstein, Gerald Weissmann. Intracellular Digestion: Lysosomes and Cellular Injury. Compr Physiol 2011, Supplement 26: Handbook of Physiology, Reactions to Environmental Agents: 603-613. First published in print 1977. doi: 10.1002/cphy.cp090139