Comprehensive Physiology Wiley Online Library

Immunological Probes of Gastrointestinal Secretion

Full Article on Wiley Online Library


The sections in this article are:

1 Antibodies As Probes
1.1 Polyclonal Antisera
1.2 Monoclonal Antibodies
1.3 Immunocytochemistry
1.4 Immunoassay
1.5 Immunoblotting
1.6 Immunoprecipitation
1.7 Immunoaffinity Chromatography
2 Salivary Secretion
3 Gastric Secretion
3.1 Hydrochloric Acid
3.2 Pepsinogen
3.3 Intrinsic Factor
4 Pancreatic Secretion
5 Hepatobiliary Secretion
6 Colonic Secretion
7 Future Directions
7.1 Anti‐idiotypes
7.2 Immunodissection
7.3 Reverse Hemolytic Plaque Assay
7.4 Antibodies and Molecular Biology
Figure 1. Figure 1.

Schematic diagram of 3 forms of enzyme‐linked immunosorbent assay (ELISA). Ab, antibody; Ag, antigen.

Figure 2. Figure 2.

Schematic diagram of distribution of various epithelial components in human major salivary glands, based on paired immunofluorescence staining. Dotted, shaded, and black areas indicate increasing occurrence and staining intensity. SC, secretory component.

From Korsrud and Brandtzaeg 86
Figure 3. Figure 3.

Normal human gastric mucosa (cardia) stained with antibodies to (A) pepsinogen I (PG I) (X 75) and (B) pepsinogen II (PG II) (X 75) by the indirect immunoalkaline phosphatase technique.

Courtesy of Dr. Stephan Meuwissen
Figure 4. Figure 4.

Electron micrograph of secretory canalicular membrane of human parietal cell stained with antihuman intrinsic factor (IF) antibody by the indirect immunoperoxidase technique. The secretion was prepared from a gastric biopsy recovered 15 min after gastrin stimulation. Intrinsic factor is present on microvilli and on inner and outer membranes of cross‐sectioned microvillus (arrow). TV, tubulovesicles. Bar, 0.2 μm. X 50,000.

From Levine et al. 95
Figure 5. Figure 5.

Electron micrograph of rat pancreatic tissue stained with antiamylase antibody by the protein A‐gold technique. Gold particles are clustered over the zymogen granules (Z) and the flocculent material in the acinar lumen (L). A few gold particles decorate the rough endoplastic reticulum. X 26,500.

From Roth et al. 134
Figure 6. Figure 6.

Human colonic epithelium stained with anti‐carbonic anhydrase antibody by the immunoperoxidase technique. Staining is limited to nongoblet columnar cells in superficial epithelium. Bar, 100 μm. X 250.

From Spicer et al. 161

Figure 1.

Schematic diagram of 3 forms of enzyme‐linked immunosorbent assay (ELISA). Ab, antibody; Ag, antigen.

Figure 2.

Schematic diagram of distribution of various epithelial components in human major salivary glands, based on paired immunofluorescence staining. Dotted, shaded, and black areas indicate increasing occurrence and staining intensity. SC, secretory component.

From Korsrud and Brandtzaeg 86

Figure 3.

Normal human gastric mucosa (cardia) stained with antibodies to (A) pepsinogen I (PG I) (X 75) and (B) pepsinogen II (PG II) (X 75) by the indirect immunoalkaline phosphatase technique.

Courtesy of Dr. Stephan Meuwissen

Figure 4.

Electron micrograph of secretory canalicular membrane of human parietal cell stained with antihuman intrinsic factor (IF) antibody by the indirect immunoperoxidase technique. The secretion was prepared from a gastric biopsy recovered 15 min after gastrin stimulation. Intrinsic factor is present on microvilli and on inner and outer membranes of cross‐sectioned microvillus (arrow). TV, tubulovesicles. Bar, 0.2 μm. X 50,000.

From Levine et al. 95

Figure 5.

Electron micrograph of rat pancreatic tissue stained with antiamylase antibody by the protein A‐gold technique. Gold particles are clustered over the zymogen granules (Z) and the flocculent material in the acinar lumen (L). A few gold particles decorate the rough endoplastic reticulum. X 26,500.

From Roth et al. 134

Figure 6.

Human colonic epithelium stained with anti‐carbonic anhydrase antibody by the immunoperoxidase technique. Staining is limited to nongoblet columnar cells in superficial epithelium. Bar, 100 μm. X 250.

From Spicer et al. 161
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Adam Smolka. Immunological Probes of Gastrointestinal Secretion. Compr Physiol 2011, Supplement 18: Handbook of Physiology, The Gastrointestinal System, Salivary, Gastric, Pancreatic, and Hepatobiliary Secretion: 327-344. First published in print 1989. doi: 10.1002/cphy.cp060317