Comprehensive Physiology Wiley Online Library

Application of Tissue Culture Techniques to Study of Renal Tubular Epithelia

Full Article on Wiley Online Library



Abstract

The sections in this article are:

1 Types of Renal Epithelial Tissue Cultures
1.1 Continuous Lines from Dispersed Cells
1.2 Primary Cultures
1.3 Continuous Lines from Dissected Renal Epithelia
1.4 Immortalization of Renal Epithelial Cells
2 Epithelial Cell Biology
2.1 Plasma Membrane Polarity
2.2 Intercellular Junctions
2.3 Junctional Complexes
2.4 Gap Junctions
2.5 Differentiation
3 Control of Renal Epithelial Cell Growth
3.1 Composition of the Medium
3.2 Substrata
4 Transport
4.1 Transepithelial Transport and the Formation of Domes
4.2 Transcytosis
4.3 Sodium, Potassium, and Chloride Fluxes
4.4 Na+,K+−ATPase
4.5 Cellular Electrophysiology
4.6 Cellular pH Regulation
4.7 Regulation of Cell Calcium
4.8 Sodium‐Coupled Hexose Transport
4.9 Sodium‐Coupled Phosphate Transport
4.10 Amino Acid Transport
4.11 Adjustment to Altered Osmolality
5 Effects of Hormones on Cultured Epithelia
5.1 Adenosine
5.2 Adrenergic Agents
5.3 Aldosterone
5.4 Atrial Natriuretic Factor
5.5 Calcitonin
5.6 Glucagon
5.7 Insulin
5.8 Parathyroid Hormone
5.9 Prostaglandins
5.10 Vasopressin
5.11 Activators of Protein Kinase C
6 Somatic Cell Genetics
7 Conclusions
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Joseph S. Handler, Maurice B. Burg. Application of Tissue Culture Techniques to Study of Renal Tubular Epithelia. Compr Physiol 2011, Supplement 25: Handbook of Physiology, Renal Physiology: 385-414. First published in print 1992. doi: 10.1002/cphy.cp080110